Plate 1 a,b. Comparison of the stomach mucosa of rats fed with raw GM potato diet (b) shows marked thickening due to hypertrophy of mucosal cells in comparison with that of rats given the parental line (a) (7 mm bar = 100 μm).
Plate 2 a,b,c,d. Histology of the jejunum and ileum of rats fed raw GM or parent potato diets. Jejunal crypt length and cells exhibit marked enlargement after feeding rats GM potato diets for 10 days (b) in comparison with that of rats given parental line potato diets (a). The villus length is similar in both but intraepithelial lymphocyte cell counts appear to be increased on GM potato diet. In the ileum both crypts and villi of rats on GM potato diets are elongated (d) in comparison with parent potato-fed rats (c) (14 mm bar = 100 μm).
Plate 3 a,b,c,d. The mucosa of caecum demonstrates little change. Differences between GM-fed (b) and parent line potato-fed rats (a) are slight, while the colonic mucosa is moderately thickened in GM-fed rats (d) compared with that of rats given the parental line (c) (14 mm bar + 50 μm).
Plate 4 a,b. Immunocytochemistry of the jejunum of rats fed raw GM potato diets for 10 days (a) shows moderate binding of GNA to villus tips (14 mm bar = 50 μm). Similar binding of GNA to jejunal villus tips is found in rats given parent potato diet supplemented with GNA in amounts equivalent to that expressed in the GM potato (b). Sections were first treated with anti-GNA rabbit antibody (1/100 diluted), followed by visualization with PAP. Note the strong antibody reactivity of feed particles in the sections.